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All mice look pretty similar, so you might not know if you have a male or female. The process of determining sex is called sexing. There are two main methods of determining sex; both require a keen eye and gentle handling of mice. You can sex mice by looking at the anogenital distance, which is the distance between the mouse's genital area and anus. Another method for sexing young mice is to check to see if the nipples are visible. It's always best to know the sex of your mice so you can separate the males from the females and prevent unwanted pregnancies.
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Scientists just created healthy mice with same-sex parents

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Switching a Gene in Adult Mice Easily Transforms Females Into Males | Popular Science

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. A Nature Research Journal.
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Switching a Gene in Adult Mice Easily Transforms Females Into Males

We hypothesized that a significant portion of sexual differentiation of mouse external genitalia occurs postnatally. To establish a baseline of normal development to which genital abnormalities could be compared, we determined morphometric measurements and morphological descriptions of the postnatal period of sexual differentiation in mice from birth to 21 days postnatally. Mice were studied by age group, including ages 0 to 1, 2 to 3, 4 to 5, 10 and 21 days. Three-dimensional reconstructions were created to visually compare differences.
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In female mammals, it remains controversial whether maternal diet and particularly the source and availability of energy can influence sex of offspring born. Mice delivered four litters of pups, resulting in a total of 1, young born over pregnancies. Gestation length and litter size did not differ between VHF and LF groups and did not change as mice aged.
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